联合检测Shh Gli1 Snail及E-cadherin在胃癌中的表达及其意义

郝亚琴; 欧阳小波; 王立

doi:10.3969/j.issn.1000-8179.2011.07.005

摘要: 目的：探讨Shh、 Gli1、 Snail及E-cadherin在胃癌中的表达及意义。方法：采用免疫组化方法检测54例胃癌组织、癌旁组织及30例正常人胃黏膜组织中Shh、 Gli1、 Snail及E-cadherin的表达情况，并与临床病理资料做对照分析。结果：胃癌组织中Shh、 Gli1、 Snail及E-cadherin阳性表达率分别为68.52%、 61.11%、 77.78%、 25.93%；癌旁组织27.78%、 24.07%、 40.74%、 70.37%；正常胃粘膜组织36.67%、 26.67%、 30.00%、 96.67%。四种蛋白在胃癌组织和癌旁组织中的表达差异均具有统计学意义（P<0.05），Shh、 Gli1及Snail在癌旁组织及正常胃黏膜组织中的表达差异均无统计学意义（P>0.05）， E-cadherin有统计学意义（P<0.05）；四种蛋白的表达均与胃癌的分化程度、淋巴结转移相关（P<0.05），其中Shh、 Gli1及Snail与胃癌浸润深度有关（P<0.05），而E-cadherin无关（P>0.05）；四种蛋白的表达与性别、年龄、位置及远处转移均无相关性（P>0.05）； Shh、 Gli1和Snail三者之间在胃癌中的表达呈正相关（P<0.05），而这三个蛋白表达与E-cadherin呈负相关（P<0.05）。结论： Hedgehog信号通路与上皮间质转换（epitheli?al-mesenchymal transition， EMT）分子在胃癌组织中皆异常表达，提示异常激活的Hedgehog信号通路可能引起EMT分子的异常表达。这在胃癌的发生发展中可能起到一定的作用。

Abstract: Combined Detection of Shh, Gli1, Snail and E-cadherin Expression in Gastric Cancer and ItsSignificanceYaqin HAO, Xiaobo OUYANG, LiWANGCorrespondence to: LiWANG, E-mail: wangli380@yahoo.com.cnDepartment of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaAbstract Objective: To investigate the expression of Shh, Gli1, Snail and E-cadherin in gastric cancer ( GC ) and its signifi-cance. Methods: Immunohistochemistry was used to detect the expression of Shh, Gli1, Snail and E-cadherin proteins in 54 cases ofGC tissues, their adjacent tissues and 30 cases of normal human gastric mucosa tissues. The correlation of Shh, Gli1, Snail and E-cad-herin expression with the clinicopathologic features of GC was analyzed. Results: The positive rate of Shh, Gli1, Snail and E-cadherinexpression was 68.52%, 61.11%, 77.78% and 25.93% in GC tissues, 27.78%, 24.07%, 40.74% and 70.37% in the paraneoplastic tis-sues, and 36.67%, 26.67%, 30% and 96.67% in the normal human gastric mucosa, with a statistical significance between GC tissuesand paraneoplastic tissues ( P < 0.05 ). There was no statistical difference in the expression of Shh, Gli1 and Snail between paraneoplas-tic tissues and normal gastric mucosa ( P > 0.05 ), while the difference in the E-cadherin expression was statistically significant ( P <0.05 ). The expression of these four proteins was correlated with tumor differentiation and lymph node metastasis ( P < 0.05 ). The ex-pression of Shh, Gli1 and Snail was related to the depth of invasion, while the E-cadherin expression was not ( P > 0.05 ). The expres-sion of the above four proteins was not correlated with sex, age, location or distant metastasis ( P > 0.05 ). The expression of Shh, Gli1and Snail was positively correlated with each other in GC ( P < 0.05 ), but was negatively correlated with E-cadherin ( P < 0.05 ). Con-clusion: The expression of molecules involved in Hedgehog signaling pathway and Epithelial- mesenchymal transition (EMT) is abnor-mal in GC. The abnormally activated Hedgehog signaling pathway may lead to the abnormal expression of EMT related molecules andthis mechanism may play a certain role in the oncogenesis and progression of gastric cancer.Keywords Gastric cancer; Hedgehog signaling pathway; Snail; E-cadherin; Epithelial-mesenchymal transition